Characterization of microsomal aniline hydroxylase of rainbow trout, Salmo gairdneri.
Indian J Biochem Biophys
;
1998 Feb; 35(1): 11-5
Article
in English
| IMSEAR
| ID: sea-28761
ABSTRACT
Aniline hydroxylase from liver microsomes of rainbow trout Salmo gairdneri converted aniline to p-aminophenol, the specific activity being 0.068 nmoles/min/mg protein in potassium phosphate buffer, pH 7.4 at 25 degrees C. The maximal rate of the enzyme reaction was found at aniline concentrations above 5 mM and in the presence of NADPH. Vmax and K(m) were 0.048 nmoles/min/mg and 0.105 mM respectively. The Hill plot showed the Hill constant to be 1.02 indicating one substrate binding site with no cooperativity. Ca2+ and Mg2+ at concentrations ranging between 1-10 mM stimulated the enzyme activity.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Microsomes, Liver
/
Aniline Hydroxylase
/
Oncorhynchus mykiss
/
Animals
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1998
Type:
Article
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