Urate oxidase electrode based on dissolved oxygen probe for urine uric acid determination.
Indian J Biochem Biophys
;
2000 Feb; 37(1): 67-70
Article
in English
| IMSEAR
| ID: sea-28839
ABSTRACT
A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 1.7.3.3) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35 degrees C. The enzyme electrode response depends linearly on uric acid concentration between 5-40 microM with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oxygen
/
Urate Oxidase
/
Uric Acid
/
Humans
/
Biosensing Techniques
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2000
Type:
Article
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