Stabilization of prostaglandin synthetase by immobilization of goat seminal microsomes on silica gel-G.
Indian J Biochem Biophys
;
1990 Apr; 27(2): 76-80
Article
in English
| IMSEAR
| ID: sea-28845
ABSTRACT
Prostaglandin synthetase was immobilized by adsorption of goat vesicular microsomes on silica gel containing CaSO4 (silica gel G). Repeated cycles of enzymatic conversion of arachidonic acid to prostaglandin by the immobilized microsomes increased the product yield by 1.5 fold, in comparison to the same by free microsomal particles. The presence of Ca2+ in silica gel is responsible for this improved yield of prostaglandin as the divalent metal ion stabilized prostaglandin synthetase activity in a remarkable way. Microsomal particles immobilized on solid supports like alumina G and controlled pore glass were not very effective.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Semen
/
Enzyme Stability
/
Male
/
Goats
/
Prostaglandin-Endoperoxide Synthases
/
Silicon Dioxide
/
Enzymes, Immobilized
/
Gels
/
Animals
/
Microsomes
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1990
Type:
Article
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