Detection and estimation of 3,4-dehydroproline.
Indian J Biochem Biophys
;
1989 Apr; 26(2): 126-8
Article
in English
| IMSEAR
| ID: sea-28862
ABSTRACT
A method has been described for the detection and estimation of 3,4-dehydroproline using initial oxidation with isatin or H2O2 and subsequent reaction with p-dimethylamino benzaldehyde (Ehrlich reagent). The method is sensitive enough to detect as low as 0.6 micrograms of 3,4-dehydroproline/sq. cm on paper chromatogram. 3,4-dehydroproline could also be estimated by the classical H2O2-oxidation procedure employed for hydroxyproline apparently yielding the same chromophore on a molar basis. However, when estimated by the chloramine-T oxidation method, its sensitivity was only 1/100th of that of 4-hydroxyproline. The usefulness of these procedures in the detection and estimation of 3,4-dehydroproline has been described.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Proline
/
Chemistry
/
Chromatography, Paper
/
Chemical Phenomena
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1989
Type:
Article
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