Changes in the cellular composition of Candida albicans resistant to miconazole.
Indian J Biochem Biophys
;
1996 Oct; 33(5): 420-4
Article
in English
| IMSEAR
| ID: sea-28896
ABSTRACT
Biochemical changes that accompany acquisition of miconazole resistance in a single step mutant of C. albicans 3153 were analyzed. Experiments show that resistance to this drug was associated with decrease in total lipids, phospholipids and sterol content. Fluorescence polarization studies with 1,6-diphenyl-1,3,5-hexatriene (DPH) showed decrease in polarization value (p) in resistant cells, thus indicating changes in membrane fluidity. Uptake of [3H] proline by intact cells revealed decrease in K(m) and Vmax of high affinity system (S1) of proline transport in cells resistant to miconazole. Results of this study suggest that membrane sensitivity of miconazole is determined by overall membrane organisation rather than by affinity of antifungal drug(s) for a single membrane component.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Candida albicans
/
Drug Resistance, Microbial
/
Proline
/
Lipid Metabolism
/
Membrane Fluidity
/
Miconazole
/
Mutation
/
Antifungal Agents
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1996
Type:
Article
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