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Differences in the levels of aminoacylation and contents of modified nucleotides between total tRNAs from N2- and NH4(+)-grown Azospirillum lipoferum cells.
Indian J Biochem Biophys ; 1994 Dec; 31(6): 454-8
Article in English | IMSEAR | ID: sea-29087
ABSTRACT
Total tRNAs isolated from N2- and NH4(+)-grown Azospirillum lipoferum cells were compared with respect to amino acid acceptance, isoacceptor tRNA species levels and extent of nucleotide modifications. Amino-acylation of these two tRNA preparations with ten different amino acids indicated differences in the relative acceptor activities. Comparison of aminoacyl-tRNA patterns by RPC-5 column chromatography revealed no qualitative differences in the elution profiles. However, quantitative differences in the relative amounts of some isoacceptors were observed. These results indicate that alterations of relative amounts of functional tRNA species occur to match cellular requirements of the bacterial cells using N2 or NH4+ as nitrogen source. In addition, the content of modified nucleotides in total tRNAs of N2- and NH4(+)-grown cells was determined. In the NH4(+)-grown cells, content of most of the modified nucleotides decreased significantly. Based upon these results, the relationship of chargeability of tRNAs to base modifications is discussed.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: RNA, Transfer / Acylation / RNA Processing, Post-Transcriptional / Azospirillum / Amino Acids / Quaternary Ammonium Compounds / Nitrogen / Nucleotides Type of study: Qualitative research Language: English Journal: Indian J Biochem Biophys Year: 1994 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: RNA, Transfer / Acylation / RNA Processing, Post-Transcriptional / Azospirillum / Amino Acids / Quaternary Ammonium Compounds / Nitrogen / Nucleotides Type of study: Qualitative research Language: English Journal: Indian J Biochem Biophys Year: 1994 Type: Article