Studies on the effect of various parameters on crotalarin-fetuin interaction.
Indian J Biochem Biophys
;
1991 Apr; 28(2): 146-9
Article
in English
| IMSEAR
| ID: sea-29113
ABSTRACT
With a view to optimise the interaction of crotalarin, a blood group A-specific lectin from the seeds of Crotalaria striata with fetuin the effect of various parameters on the reaction has been studied turbidimetrically. The formation of crotalarin-fetuin complex was dependent on time, temperature, pH and the ionic strength of the medium. The maximum turbidity appeared in 30 min at 20 degrees C and the pH optimum was 3.5. The binding constant (Ka) for crotalarin-fetuin interaction was 5.58 x 10(4) M-1 (pH 3.5) at 20 degrees C. Among the different inorganic salts tested, the cations with increasing concentrations had pronounced effect on binding. KCNS and KI, however, were noninhibitory. The turbidity slightly increased in presence of different sodium salts, whereas periodate and urea reduced the interaction. The different alcohols had no remarkable effect on the above reaction.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Seeds
/
Thermodynamics
/
Kinetics
/
Alpha-Fetoproteins
/
Plant Lectins
/
Hydrogen-Ion Concentration
/
Lectins
/
Nephelometry and Turbidimetry
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1991
Type:
Article
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