Comparative study between molecular hybridization and electron microscopy for the detection of hepatitis E virus.

Soe, S; Nakane, P K; Myat, P K; Iida, F; Kyi, K P; Swe, T

Soe, S; Nakane, P K; Myat, P K; Iida, F; Kyi, K P; Swe, T.

Southeast Asian J Trop Med Public Health ; 1993 Sep; 24(3): 477-9

Article in English | IMSEAR | ID: sea-30623

ABSTRACT

ABSTRACT

The study describes a comparison between molecular hybridization using a non-radiolabeled, thymine-thymine (TT) dimerized synthetic oligonucleotide complementary DNA probe and electron microscopy for the detection of hepatitis E virus genome in bile. Spot hybridization with the TT dimerized probe was found to be more sensitive and specific compared to electron microscopy.

Subject(s)

Animals; Bile/microbiology; DNA, Complementary; DNA, Viral; Genome, Viral; Hepatitis E/microbiology; Hepatitis E virus/genetics; Microscopy, Electron/methods; Monkey Diseases/microbiology; Nucleic Acid Hybridization/methods; Oligonucleotide Probes; Pyrimidine Dimers; Sensitivity and Specificity; Time Factors

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pyrimidine Dimers / Time Factors / Bile / DNA, Viral / Microscopy, Electron / Oligonucleotide Probes / Sensitivity and Specificity / Hepatitis E virus / Hepatitis E / Genome, Viral Type of study: Diagnostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1993 Type: Article

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