Detection of blood stage antigens of Plasmodium vivax by sandwich ELISA using pan-species monoclonal antibodies and polyclonal antibodies.
Southeast Asian J Trop Med Public Health
;
1991 Sep; 22(3): 393-6
Article
in English
| IMSEAR
| ID: sea-30709
ABSTRACT
This paper reports an improved PcAb-McAb-ELISA test to detect blood stage Plasmodium vivax antigen in which the plates were coated with rabbit anti-P. cynomolgi polyclonal antibody to capture the antigens in test samples and two monoclonal antibodies, M26-32 and 3F9, were added together to react with the captured antigens. The coincidence rate with this test was 93% with microscopically confirmed P. vivax cases, 97% with normal samples, 95% with microscopically negative fever cases from nonendemic areas and 86% from endemic areas, respectively. The sensitivity was greater than 1 parasite/10(5) RBC.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmodium vivax
/
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Protozoan
/
Sensitivity and Specificity
/
Malaria, Vivax
/
Evaluation Studies as Topic
/
False Negative Reactions
/
False Positive Reactions
/
Microscopy
Type of study:
Diagnostic study
/
Evaluation studies
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1991
Type:
Article
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