Production and purification of Burkholderia pseudomallei BipD protein.
Southeast Asian J Trop Med Public Health
;
2008 Jan; 39(1): 109-14
Article
in English
| IMSEAR
| ID: sea-30834
ABSTRACT
Type III secretion system (TTSS) clusters contain virulent genes of both animal and plant Gram-negative bacteria. The full length (933 bp) gene of bipD, a member of TTSS cluster of Burkholderia pseudomallei, was isolated by PCR amplification from B. pseudomallei DNA and cloned into pGEX 4T-1. GST-BipD fusion protein and BipD protein obtained by removal of GST using thrombin were employed to detect the presence of B. pseudomallei antibodies in sera obtained from melioidosis and non-melioidosis patients by immunoblotting. Sensitivity and specificity of BipD protein was 100% and 91.1%, respectively, whereas that of fusion protein was 77.8% and 90.0%, respectively.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thailand
/
Bacterial Proteins
/
Virulence
/
Humans
/
Molecular Sequence Data
/
Case-Control Studies
/
Multigene Family
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Amino Acid Sequence
Type of study:
Diagnostic study
/
Observational study
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2008
Type:
Article
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