Rapid detection of apolipoprotein E genotypes in Alzheimer's disease using polymerase chain reaction-single strand conformation polymorphism.
Southeast Asian J Trop Med Public Health
;
2006 Jul; 37(4): 793-7
Article
in English
| IMSEAR
| ID: sea-31064
ABSTRACT
Apolipoprotein E (APOE) gene on chromosome 19q13.2 is encoded by three common alleles designated as epsilon2, epsilon3 and epsilon4. In Alzheimer's disease (AD) the epsilon4 allele is over-represented and is considered to be a major genetic risk factor. Several methods have been developed to determine APOE genotypes. Among them, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) appears to be highly reliable. In this study, we improved the nonisotopic PCR-SSCP method for determining APOE genotypes in 42 cases of AD patients, 40 cases of non-AD dementia patients, and 49 cases of age-matched controls. DNA from the target sequence on APOE was amplified by PCR from peripheral blood genomic DNA. PCR products were electrophoresed in a non-denaturing polyacrylamide gel and visualized by silver staining. We found that the epsilon4 allele had a significantly high frequency of occurrence in AD patients (33.3%) compared with age-matched controls (13.3%) (chi(2) = 10.43, p = 0.001) and non-AD dementia (10%) (chi(2) = 13.02, p<0.001) whereas the epsilon3 allele was of high frequency in non-AD dementia (90%) compared with age-matched controls (85.7%) and AD patients (66.7%). APOE epsilon4 homozygotes were found only in AD groups. On the other hand, the epsilon2 allele was found only in an age-matched control. This study confirmed that the APOE psilon4 allele is a risk factor in Thai AD subjects and that the PCR-SSCP method is a rapid and useful means of detecting the APOE genotype in AD.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Apolipoproteins E
/
Aged, 80 and over
/
Aged
/
Female
/
Humans
/
Male
/
Polymerase Chain Reaction
/
Genetic Techniques
/
Polymorphism, Single-Stranded Conformational
/
Alzheimer Disease
Type of study:
Diagnostic study
/
Risk factors
Limits:
Aged80
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2006
Type:
Article
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