Production specific antigens from Trichinella spiralis using a continuous elution method and isoelectric focusing.
Southeast Asian J Trop Med Public Health
;
1997 ; 28 Suppl 1(): 99-106
Article
in English
| IMSEAR
| ID: sea-31303
ABSTRACT
Immunodominant antigens of 45-53 kDa (one band per fraction) were obtained from excretory/secretory (E/S) and somatic products of infective-larvae of Trichinella spiralis using a continuous-elution method. They were further resolved by isoelectric focusing into different isoforms (45 kDa pI4.47, 5.09, 5.47 and 5.86; 47 kDa pI4.72 and 4.97; 53 kDa pI4.86, 5.11, 5.44 and 5.78). In immunoblotting, the isoforms of pI 5.09, 5.86, 4.97, 5.44 and 5.78 did not cross-react with antisera against Trichuris suis, Metastrongylus apri, Gnathostoma hispidum and Stephanurus dentatus. Hence, they have the potential to serve as specific antigens for the serodiagnosis of trichinellosis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Swine
/
Swine Diseases
/
Trichinellosis
/
Immunoblotting
/
Immunodominant Epitopes
/
Trichinella spiralis
/
Cross Reactions
/
Electrophoresis, Polyacrylamide Gel
/
Isoelectric Focusing
/
Larva
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1997
Type:
Article
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