Increase in the sensitivity of dengue diagnosis by combination of reverse transcriptase-polymerase chain reaction and passage on cell cultures.
Southeast Asian J Trop Med Public Health
;
2001 Sep; 32(3): 470-1
Article
in English
| IMSEAR
| ID: sea-31346
ABSTRACT
We passaged 52 serum samples from dengue patients on C6/36 cells for 7 days and checked the culture fluids by RT-PCR. Two serum samples, which were negative by direct RT-PCR, became positive. One sample was collected on fever day 1 and the other on fever day 2. Results indicate that combination of reverse transcriptase-polymerase chain reaction (RT-PCR) with passage of serum samples on C6/36 cells increases the sensitivity of dengue diagnosis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Serial Passage
/
Cell Line
/
Sensitivity and Specificity
/
Reverse Transcriptase Polymerase Chain Reaction
/
Dengue
/
Japan
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2001
Type:
Article
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