Western immunoblot analysis using a ten leptospira serovars combined antigen for serodiagnosis of leptospirosis.
Southeast Asian J Trop Med Public Health
;
2006 Mar; 37(2): 309-11
Article
in English
| IMSEAR
| ID: sea-31447
ABSTRACT
Leptospirosis is a major zoonotic disease throughout the world. There are unavailable accuracy diagnostic methods for the acute phase of infection. To demonstrate the advantage of Western immunoblot, a mixed leptospira serovars antigen for the serodiagnosis of leptospirosis was employed. SDS-PAGE and Western immunoblot was performed using a 10 mixed leptospira serovars antigen and stained with 16 reference rabbit anti-leptospirosis antibodies. The result showed different immunoreactive band patterns for each reference serum. The bands with molecular weights of 15-20, 23-24, 41 and 45 kDa were commonly found (88% to 100% of the 16 reference sera). Using combined leptospira antigens in a Western immunoblot technique is an alternative and practical strategy for a more sensitive leptospirosis serodiagnosis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Serologic Tests
/
Blotting, Western
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Electrophoresis, Polyacrylamide Gel
/
Leptospira
/
Leptospirosis
/
Molecular Weight
/
Antibodies, Bacterial
Type of study:
Diagnostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2006
Type:
Article
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