Comparison of two in vitro sensitivity tests for Plasmodium falciparum.
Southeast Asian J Trop Med Public Health
;
2006 Jan; 37(1): 5-12
Article
in English
| IMSEAR
| ID: sea-31468
ABSTRACT
The main purpose of the study was to compare the in vitro sensitivity results obtained from the two widely-used in vitro systems (1) standard WHO micro-technique based on schizont maturation inhibition using fresh isolates (M-I), and (2) micro-technique based on incorporation of [3H]-hypoxanthine using culture-adapted isolates (M-II). The study was conducted during 1998 and 2002. A total of 473 Plasmodium falciparum isolates were collected from five highly malaria endemic areas of Thailand, ie, Mae Sot district, Tak (north-western), Kanchanaburi (western), Ranong (south-western), Ratchaburi (south-western) and Chantaburi (eastern) Provinces. The antimalarials tested were mefloquine, quinine, chloroquine, artemisinin and dihydroartemisinin. The sensitivity results for mefloquine obtained from the two methods were significantly different from each other. The IC50 values for M-II was less than M-I. The median (95%C.I.) IC50 value for mefloquine using the M-II method was significantly lower [696.47 (393.11-1,233.2) nM] than for M-I [3,955.4 (1,035.61-5,108.9) nM]. The in vitro sensitivity results for quinine were significantly different from each other. The median (95% C.I.) IC50 value for M-II [161 (42-351) nM] was 2.5-fold that of M-I [66 (24-450) nM].
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmodium falciparum
/
Humans
/
Inhibitory Concentration 50
/
Parasitic Sensitivity Tests
/
Animals
/
Antimalarials
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2006
Type:
Article
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