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Detection of respiratory syncytial virus from clinical specimens: comparison between reverse transcription polymerase chain reaction and tissue culture.
Southeast Asian J Trop Med Public Health ; 1995 Dec; 26(4): 684-8
Article in English | IMSEAR | ID: sea-31506
ABSTRACT
RT-PCR was compared with tissue culture to detect RSV from nasopharyngeal aspirates. RT-PCR was more sensitive and specific than tissue culture method. RT-PCR has sensitivity and specificity of 100% and 97%, respectively. The results indicate that RT-PCR can be used for detection of RSV in clinical specimens.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Virus Cultivation / Humans / Infant, Newborn / RNA, Viral / Blotting, Southern / Reproducibility of Results / Sensitivity and Specificity / Respiratory Syncytial Virus, Human / Random Amplified Polymorphic DNA Technique / Culture Techniques Type of study: Diagnostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1995 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Virus Cultivation / Humans / Infant, Newborn / RNA, Viral / Blotting, Southern / Reproducibility of Results / Sensitivity and Specificity / Respiratory Syncytial Virus, Human / Random Amplified Polymorphic DNA Technique / Culture Techniques Type of study: Diagnostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1995 Type: Article