Attempt to culture Wuchereria bancrofti in vitro.
Southeast Asian J Trop Med Public Health
;
1986 Dec; 17(4): 579-81
Article
in English
| IMSEAR
| ID: sea-31637
ABSTRACT
Third-stage larvae of Wuchereria bancrofti recovered from laboratory raised Aedes togoi and Anopheles maculatus fed on a human volunteer were recovered by mass dissection methods and introduced into in vitro culture. LLC-MK2 cells were used as feeder cells, and the culture medium consisted of RPMI-1640 buffered with Hepes and sodium bicarbonate and supplemented with human AB serum. The third-stage larvae molted as early as 12 days and those surviving had all molted by 16 days. The fourth-stage parasites averaged in length from 1.4 mm to a maximum of 1.8 mm. Some larvae remained alive in culture as long as 40 days and while the worms were distorted in fixation, possible primodial cells of a spicule could be visualized in the rectal region. The cuticle also appeared to be separating in the posterior end. Although complete development was not achieved, it seems that with a continuing effort, success could be obtained using this culture system with feeder cells.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Wuchereria
/
Wuchereria bancrofti
/
Aedes
/
Culture Media
/
Larva
/
Animals
/
Anopheles
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1986
Type:
Article
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