IgM capture ELISA for detection of IgM antibodies to dengue virus: comparison of 2 formats using hemagglutinins and cell culture derived antigens.
Southeast Asian J Trop Med Public Health
;
1992 Dec; 23(4): 726-9
Article
in English
| IMSEAR
| ID: sea-31863
ABSTRACT
The highly sensitive AFRIMS format IgM capture ELISA for the diagnosis of dengue virus infections requires the use of mouse brain derived hemagglutinins and consequently also the use of 20% acetone extracted normal human serum to eliminate high background. These reagents are not always easily available and we have thus compared the AFRIMS format with another published format which uses cell culture derived antigens (culture fluid, CF, format) in order to determine if it is reasonable to use cell culture derived antigens in situations where hemagglutinins and normal human serum are difficult to obtain. The study shows that using AFRIMS results as the reference point, the CF format described here has a sensitivity of 90% and a specificity of 96%.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Hemagglutination Inhibition Tests
/
Sensitivity and Specificity
/
Dengue
/
Dengue Virus
/
Evaluation Studies as Topic
/
Antibodies, Viral
/
Antigens, Viral
Type of study:
Diagnostic study
/
Evaluation studies
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1992
Type:
Article
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