Your browser doesn't support javascript.
loading
Discriminatory powers of molecular typing techniques for methicillin-resistant Staphylococcus aureus in a university hospital, Thailand.
Southeast Asian J Trop Med Public Health ; 2006 Mar; 37(2): 327-34
Article in English | IMSEAR | ID: sea-31999
ABSTRACT
Discriminatory powers of various molecular techniques were evaluated for typing of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Siriraj Hospital, Bangkok, Thailand. Thirty MRSA isolates were randomly selected in this study. They were characterized by pulsed-field gel electrophoresis, Clal-mecA and Clal-Tn554 polymorphisms, ribotyping, and PCR-based methods including SCCmec typing, spa and coa gene polymorphism, and repeat units in hypervariable region downstream of mecA. Individual molecular typing technique distinguished those MRSA isolates into 2 to 5 types. Eleven genetic backgrounds of MRSA isolates were elucidated by combination of typing methods with trimethoprim/sulfamethoxazole (TMP/SXT) susceptibility. Combination of all typing methods including TMP/SXT susceptibility yielded a discriminatory index of 0.94. Combination of PCR-based methods and TMP/SXT susceptibility, with the discriminatory index of 0.89, is a practical typing approach suitable for rapid epidemiological investigation of MRSA isolates in a hospital setting.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Polymorphism, Genetic / Staphylococcal Infections / Staphylococcus aureus / Thailand / Polymorphism, Restriction Fragment Length / Humans / DNA, Bacterial / Base Sequence / Microbial Sensitivity Tests / Polymerase Chain Reaction Type of study: Diagnostic study / Prognostic study Country/Region as subject: Asia Language: English Journal: Southeast Asian J Trop Med Public Health Year: 2006 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: IMSEAR (South-East Asia) Main subject: Polymorphism, Genetic / Staphylococcal Infections / Staphylococcus aureus / Thailand / Polymorphism, Restriction Fragment Length / Humans / DNA, Bacterial / Base Sequence / Microbial Sensitivity Tests / Polymerase Chain Reaction Type of study: Diagnostic study / Prognostic study Country/Region as subject: Asia Language: English Journal: Southeast Asian J Trop Med Public Health Year: 2006 Type: Article