Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera.
Southeast Asian J Trop Med Public Health
;
1998 Sep; 29(3): 579-83
Article
in English
| IMSEAR
| ID: sea-32070
ABSTRACT
The immunogenic components of Fasciola gigantica excretory-secretory (ES) products were revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting technic using sera from patients with F. gigantica infection, from patients with clinical suspected fascioliasis, from patients with other illness and from healthy adults. By SDS-PAGE, it was found that the ES products comprised more than 6 polypeptides. Immunoblotting analysis revealed 12 components which were strongly recognized by fascioliasis antisera. These antigenic components had a molecular mass ranging from less than 14.4 to 38 kDa. One antigenic band of 27 kDa was found to give a consistent reaction with fascioliasis antisera (100% sensitivity and 98% specificity). The present findings suggest that the 27 kDa components are sensitive and specific for the diagnosis of human F. gigantica infection.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Serologic Tests
/
Immunoblotting
/
Sensitivity and Specificity
/
Adult
/
Electrophoresis, Polyacrylamide Gel
/
Fasciola
/
Fascioliasis
/
Animals
/
Antigens, Helminth
Type of study:
Diagnostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1998
Type:
Article
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