Vaccine development against Theileria parasite.
Southeast Asian J Trop Med Public Health
;
1997 ; 28 Suppl 1(): 148-54
Article
in English
| IMSEAR
| ID: sea-32138
ABSTRACT
Bovine piroplasmosis caused by Theileria sergenti is a major cause of economical loss in grazing cattle in Japan. We found that parasite stocks and isolates consist of genetically and antigenically mixed population. To differentiate parasite populations bearing 2 allelic forms of p32, an immunodominant piroplasm surface protein, 2 sets of oligonucleotide primers were designed to amplify either of the 2 alleles by polymerase chain reaction (PCR). By using this allele-specific PCR, we found that the majority of T. sergenti-infected calves in Japan harbored mixed parasite populations with C and I type parasites. Amino acid sequence of p32 contains Lys-Glu-Lys (KEK) motif which is one of tripeptide necessary for malaria parasite to invade erythrocytes. We produced 2 vaccine candidates, recombinant baculovirus p32 and synthetic peptide containing KEK motif. Immunization of either recombinant p32 or synthetic peptide containing a KEK sequence with adjuvant resulted in low parasitemia and reduced the clinical symptoms compared to control calves. Interestingly, parasites with a p32 allelic form corresponding to one used as the immunogen were suppressed. Therefore, a cocktail vaccine containing KEK peptides derived from C and I type parasites is desired for control Theileria parasite infection in Japan.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Peptide Fragments
/
Theileriasis
/
Cattle
/
Molecular Sequence Data
/
Vaccines, Synthetic
/
Protozoan Proteins
/
Immunodominant Epitopes
/
Protozoan Vaccines
/
Amino Acid Sequence
/
Vaccination
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1997
Type:
Article
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