Comparative nucleotide and deduced amino acid sequence of the envelope glycoprotein gene among three dengue virus type 2 strains isolated from patients with different disease severities in Maha Sarakham, northeast Thailand.
Southeast Asian J Trop Med Public Health
;
1994 Jun; 25(2): 243-51
Article
in English
| IMSEAR
| ID: sea-32229
ABSTRACT
The nucleotide (nt) sequence of the envelope glycoprotein (E) gene of dengue virus type 2 was determined by the primer-extension dideoxy chain-termination method for 3 dengue virus type 2 (D2) strains which had been isolated from patients with dengue fever (DF), dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS), in Maha Sarakham, Northeast Thailand, in 1986-1987. Their nt sequences were essentially the same except for a single silent nt replacement in each DHF and DSS strain compared with DF strain. Therefore, these 3 strains possessed identical deduced amino acid (AA) sequences in their E protein. The result indicated that the primary structure of the E protein of D2 virus is not related to the clinical severity of the infected patients. Eleven nt replacements which resulted in 4 amino acid replacements were found to be unique to these 3 Northeast Thai strains. Sequence similarity showed that the 3 Northeast Thai strains were closest to the DSS isolate (H) followed by the DHF isolate (D) identified in Bangkok in 1980.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thailand
/
Humans
/
RNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Viral Envelope Proteins
/
Amino Acid Sequence
/
Cloning, Molecular
/
DNA, Complementary
/
DNA Primers
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1994
Type:
Article
Similar
MEDLINE
...
LILACS
LIS