The use of polymerase chain reaction (PCR) as a diagnostic tool for dengue virus.
Southeast Asian J Trop Med Public Health
;
1995 Dec; 26(4): 669-72
Article
in English
| IMSEAR
| ID: sea-32364
ABSTRACT
This study describes the use of polymerase chain reaction as a diagnostic tool for detecting and typing of dengue virus. PCR was compared against virus isolation. First RT-PCR was done using dengue consensus primers after which positive samples were subjected to RT-PCR using type-specific primers. This study shows that the local strains of the dengue virus could be detected using the chosen primers. Furthermore, RT-PCR was found to be more sensitive than virus isolation in identifying the dengue positive samples.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
RNA, Viral
/
Serotyping
/
Case-Control Studies
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Random Amplified Polymorphic DNA Technique
/
Dengue Virus
/
Malaysia
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1995
Type:
Article
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