Comparative study on rapid dot-immunogold staining and two immunogold silver staining assays for diagnosing schistosomiasis japonica.
Southeast Asian J Trop Med Public Health
;
2005 Jan; 36(1): 79-82
Article
in English
| IMSEAR
| ID: sea-32493
ABSTRACT
A fast, specific, sensitive, convenient, and economical rapid-dot-immunogold staining (R-Dot-IGS) assay was used to detect serum antibodies in patients infected with Schistosoma japonicum. The soluble egg antigen of Schistosoma japonicum was added onto microspore membrane. After pre-reacting and blocking, the serum to be detected and sheep anti-human IgG labeled with chloroauric acid were added sequentially. The assay took 15 minutes. For comparison, the dot-immunogold silver staining (Dot-IGSS) and rapid micro-volume Dot-IGSS (RM-Dot-IGSS) assay were also performed. The positive rate to detect the serum of schistosomiasis japonica by the R-Dot-IGS, Dot-IGSS and RM-Dot-IGSS assay was 98%, 98% and 100%, respectively. Samples from 50 healthy controls, 10 cases of clonorchiasis, and 10 cases of paragonimiasis showed negative reactions except for one case of clonorchiasis with RM-Dot-IGSS assay. Compared with Dot-IGSS and RM-Dot-IGSS, R-Dot-IGS assay has similar sensitivity and specificity, but the latter is quicker, simpler, and cheaper. Therefore, R-Dot-IGS is strongly recommended for rapid diagnosis of schistosomiasis japonica both in epidemiological study and in the clinic.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Schistosoma japonicum
/
Staining and Labeling
/
Schistosomiasis japonica
/
Humans
/
Antibodies, Helminth
/
Immunohistochemistry
/
Immunoblotting
/
Case-Control Studies
/
Sensitivity and Specificity
/
Silver Staining
Type of study:
Diagnostic study
/
Observational study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2005
Type:
Article
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