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A comparison of the ELISA with other serological tests in the serodiagnosis of amoebiasis.
Southeast Asian J Trop Med Public Health ; 1989 Sep; 20(3): 399-405
Article in English | IMSEAR | ID: sea-32571
ABSTRACT
The sera of 78 patients with invasive amoebiasis were tested for antiamoebic antibodies by the techniques of enzyme-linked immunosorbent assay (ELISA), indirect haemagglutination (IHA), indirect fluorescent antibody (IFA) and counterimmunoelectrophoresis (CIEP). Results showed that the ELISA compared favourably with IHA and IFA tests in terms of sensitivity and specificity. ELISA, IHA and IFA detected 97.4%, 96.2% and 98.7% of the patients respectively. CIEP was the least sensitive of the 4 serological methods with a sensitivity of 88.5%. The advantages and disadvantages of the 4 serodiagnostic procedures are discussed.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Humans / Immunoglobulin G / Counterimmunoelectrophoresis / Enzyme-Linked Immunosorbent Assay / Hemagglutination Tests / Serologic Tests / Antibodies, Protozoan / Predictive Value of Tests / Fluorescent Antibody Technique / Amebiasis Type of study: Diagnostic study / Prognostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1989 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Humans / Immunoglobulin G / Counterimmunoelectrophoresis / Enzyme-Linked Immunosorbent Assay / Hemagglutination Tests / Serologic Tests / Antibodies, Protozoan / Predictive Value of Tests / Fluorescent Antibody Technique / Amebiasis Type of study: Diagnostic study / Prognostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1989 Type: Article