Detection of Angiostrongylus malaysiensis circulating antigen using monoclonal antibody-based enzyme-linked immunosorbent assay (MAb-ELISA).
Southeast Asian J Trop Med Public Health
;
1997 ; 28 Suppl 1(): 143-7
Article
in English
| IMSEAR
| ID: sea-32637
ABSTRACT
Three MAbs 1C4.2D8, 1C4.2C4 and 1C4.1F5 were produced using sonicated adult worm antigens of Angiostrongylus malaysiensis and they were found to be secreters of IgG1. The MAbs 1C4.2C4 and 1C4.2D8 were found to react with antigens of A. malaysiensis and cross-react with the closely related A. cantonensis but not with other helminths. A total of 108 human sera collected from Orang Asli (aborigenes) from Grik, in the State of Perak were tested for A. malaysiensis infection using the MAb-ELISA. MAb 1C4.1F5 and 25 (23%) were positive. Twenty of these positive samples were tested with the MAb 1C4.2D8 and none was found to be positive.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Strongylida Infections
/
Cross Reactions
/
Racial Groups
/
Native Hawaiian or Other Pacific Islander
/
Angiostrongylus
/
Animals
/
Malaysia
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1997
Type:
Article
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