Comparison of the diagnostic value of local Widal slide test with imported Widal slide test.
Southeast Asian J Trop Med Public Health
;
2004 Jun; 35(2): 366-70
Article
in English
| IMSEAR
| ID: sea-32955
ABSTRACT
A laboratory study comparing the Widal slide agglutination test using local antigens produced by Mekar Jaya Diagnostica (SAT-MJD) with imported antigens (Murex, Abbott) was carried out on 55 sera of typhoid fever patients with positive blood culture and 56 sera of non-typhoid febrile patients. The SAT-MJD antigens consisted of a mixture of 5 different phage-types of S. typhi dominantly found in Indonesia. This study revealed the following results:
the diagnostic sensitivity of local and imported antigens was 83.93%, the diagnostic specificity of local antigens was higher than the imported antigens ie 82.14% compared with 64.28%, the diagnostic efficiency of local antigens was 82.88% compared with 73.87% of the imported antigens. The diagnostic positive and negative predictive values of the local antigens were 80.70% and 83.63%, respectively. The imported antigen revealed diagnostic positive and negative predictive values of 69.69% and 80%, respectively. The diagnostic specificity and efficiency of local antigens were significantly different (p < 0.02 and p < 0.05) from the imported antigens. The local antigens have some advantages. There was no variation in within-run and between-day test, compared with a 6.6% variation shown by the imported antigen. The test results obtained 5 minutes after mixing the serum with antigens reduced the possibility of false-positive and false-negative results. The cost of local antigens is lower than the imported antigens. Based on these data, the Widal SAT-MJD has a reliable diagnostic value and can be used in small laboratories, such as primary health centers (Puskesmas).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Typhoid Fever
/
Humans
/
Agglutination Tests
/
Case-Control Studies
/
Predictive Value of Tests
/
Sensitivity and Specificity
/
Adult
/
Internationality
/
Indonesia
/
Antigens, Bacterial
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Risk factors
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2004
Type:
Article
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