Hemolysins and plasmid profiles of Vibrio parahaemolyticus.

Vadivelu, J; Puthucheary, S D; Mitin, A; Wan, C Y; van Melle, B; Puthucheary, J A

Vadivelu, J; Puthucheary, S D; Mitin, A; Wan, C Y; van Melle, B; Puthucheary, J A.

Southeast Asian J Trop Med Public Health ; 1996 Mar; 27(1): 126-31

Article in English | IMSEAR | ID: sea-32968

ABSTRACT

ABSTRACT

Forty clinical isolates of Vibrio parahaemolyticus were studied for the production of the thermostable direct hemolysin (TDH), and the TDH-related hemolysin (TRH) including the respective encoding genes, tdh and trh. The presence of TDH and its encoding genes were found amongst 95% of the strains, whereas the TRH was absent amongst these isolates. Thirty-two isolates were found to be plasmid-free, whereas eight isolates possessed plasmids with sizes ranging from 2.4 > or = 23 kb. Using a DNA probe coding for the homologous region of the tdh and trh, it was found that the tdh genes were present on the chromosomal DNA.

Subject(s)

Bacteriological Techniques; DNA Probes/diagnosis; DNA Transposable Elements/genetics; DNA, Bacterial/genetics; Food Microbiology; Foodborne Diseases/microbiology; Gastroenteritis/microbiology; Hemolysin Proteins/blood; Humans; Plasmids/genetics; Vibrio Infections/microbiology; Vibrio parahaemolyticus/genetics

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Plasmids / Vibrio Infections / Vibrio parahaemolyticus / Humans / DNA, Bacterial / DNA Transposable Elements / DNA Probes / Bacteriological Techniques / Food Microbiology / Foodborne Diseases Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1996 Type: Article

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