In vitro development of Haplorchis taichui (Trematoda: Heterophyidae).
Southeast Asian J Trop Med Public Health
;
2001 ; 32 Suppl 2(): 31-5
Article
in English
| IMSEAR
| ID: sea-33139
ABSTRACT
Newly excysted metacercariae of Haplorchis taichui were cultured in a candle jar set at 37 degrees C. Both monophasic culture media [0.85% NaCl, RPMI 1640, RPMI 1640+10% fetal calf serum (FCS)] and diphasic culture media [RPMI 1640 + egg yolk agar, RPMI 1640 + 5%, 10% or 15% blood in blood agar (BA), RMPI 1640 + 5%, 10% and 15% FCS with 5% blood in BA] were used in vitro. Parasites survived for only 1 day in 0.85% NaCl without any development. In RPMI 1640 with egg yolk agar and RMPI 1640 + 5%, 10% FCS, the parasite survived for 3-5 days. In contrast, worms survived for 12-14 days in RPMI 1640 with blood agar without any change in result in a different concentration of blood in BA. The ovary and testes were observed after 3 days incubation in this media. Nevertheless, only 1 parasite in RPMI 1640 with 15% blood in BA had vitellaria and eggs at day 6. RPMI 1640 with blood agar can be used as short-term maintenance for the in vitro culture of H. taichui. However, further studies are needed.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Temperature
/
Trematode Infections
/
Cyprinidae
/
Culture Media, Conditioned
/
Culture Media
/
Fish Diseases
/
Heterophyidae
/
Animals
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2001
Type:
Article
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