Isolation and polymerase chain reaction detection of Mycoplasma pneumoniae in Malaysian patients with respiratory tract infections.
Southeast Asian J Trop Med Public Health
;
2000 Dec; 31(4): 688-92
Article
in English
| IMSEAR
| ID: sea-33169
ABSTRACT
Isolation and polymerase chain reaction (PCR) were performed for detection of Mycoplasma pneumoniae from respiratory tract specimens obtained from 200 adult and 200 pediatric patients. M. pneumoniae was isolated from bronchoalveolar lavage fluid of 1(0.5%) adult patient and 4(2.0%) tracheal aspirates of pediatric patients. PCR was positive for only one (0.5%) broncoalveolar lavage fluid of an adult patient and fifteen (7.5%) tracheal aspirates of pediatric patients. This study suggested that M. pneumoniae was more frequently detected in pediatric patients and PCR appears to have advantages over isolation, in terms of rapidity and sensitivity.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Respiratory Tract Infections
/
Female
/
Humans
/
Male
/
Base Sequence
/
Child
/
Child, Preschool
/
Polymerase Chain Reaction
/
DNA Primers
/
Adult
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2000
Type:
Article
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