In vivo culture of Cryptosporidium oocysts for laboratory use.
Southeast Asian J Trop Med Public Health
;
2006 ; 37 Suppl 3(): 18-20
Article
in English
| IMSEAR
| ID: sea-33221
ABSTRACT
In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserved in phosphate buffered saline with antibiotic at 4 degrees C before use.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Parasite Egg Count
/
Rodent Diseases
/
Centrifugation, Density Gradient
/
Cryptosporidium
/
Culture Media
/
Oocysts
/
Culture Techniques
/
Feces
/
Animals
/
Mice
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2006
Type:
Article
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