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Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen.
Southeast Asian J Trop Med Public Health ; 1989 Mar; 20(1): 119-24
Article in English | IMSEAR | ID: sea-33379
ABSTRACT
Monoclonal antibodies (MAb) against A cantonensis were produced through fusion of immunised spleen cells from BALB/c mice with NS-1 myeloma cells at a ratio of 101. The successful fusion rate on the 3rd day of fusion was 90.1%. Ten MAb were characterised, six of which were IgG1 and the remaining four were IgG2a, IgG2b, IgM and IgA respectively. Among 6 IgG1 MAb, four were A. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile worm antigens. Two other IgG1 MAb showed cross-reaction with other helminthic antigens of Toxocara canis. Ascaris suum. Paragonimus westermani, Dirofilaria immitis, Anisakis Spp, Gnatostoma Spinigerum and Clonorchis sinensis.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Female / Enzyme-Linked Immunosorbent Assay / Hybridomas / Angiostrongylus / Animals / Metastrongyloidea / Mice / Mice, Inbred BALB C / Antibodies, Monoclonal / Antigens, Helminth Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1989 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Female / Enzyme-Linked Immunosorbent Assay / Hybridomas / Angiostrongylus / Animals / Metastrongyloidea / Mice / Mice, Inbred BALB C / Antibodies, Monoclonal / Antigens, Helminth Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1989 Type: Article