Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark.
Southeast Asian J Trop Med Public Health
;
2003 ; 34 Suppl 2(): 51-5
Article
in English
| IMSEAR
| ID: sea-33391
ABSTRACT
The in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark of Picrasma javanica B1; ie water, methanol, chloroform and hexane extracts were studied using a modification of the [3H]hypoxanthine incorporation method. It was found that the hexane extract showed in vitro antimalarial activity with IC50 of 3.3 microg/ml. The extract was further fractionated using quick column chromatography, resulting in ten fractions. Fraction V was the most effective against P. falciparum K1 with IC50 of 4.4 microg/ml. Further isolation of fraction V using a column chromatographic technique provided six fractions. According to 1H- and 13C-NMR spectra, it could be concluded that the major compound in fraction V-3 was beta-sitosterol. Unfortunately, the antimalarial activity of beta-sitosterol could not be determined because of its low solubility in DMSO. However, fractions V-2 and V-4 still showed in vitro antimalarial activities with IC50 of 2.8 and 3.4 microg/ml, respectively. The further fractionation of these two active fractions could lead to promising candidates as antimalarial agents.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmodium falciparum
/
Thailand
/
Plant Extracts
/
Magnetic Resonance Spectroscopy
/
Inhibitory Concentration 50
/
Plant Bark
/
Picrasma
/
Dose-Response Relationship, Drug
/
Animals
/
Antimalarials
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2003
Type:
Article
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