Amplification of Bcl I region of the factor VIII gene by PCR.
Southeast Asian J Trop Med Public Health
;
1996 Jun; 27(2): 364-6
Article
in English
| IMSEAR
| ID: sea-33999
ABSTRACT
A study was initiated to amplify by polymerase chain reaction (PCR), a short factor VIII gene fragment containing the Bcl I restriction site from hemophilia patients using published primer sequences. Preliminary findings indicated that the resulting fragment is 142 bp long. This fragment, when digested with Bcl I restriction enzyme produced two fragments, 99 bp and 43 bp in length. Polymorphism in the Bcl I region can be used to detect carrier state in the family members of the hemophiliacs.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Polymorphism, Genetic
/
Polymorphism, Restriction Fragment Length
/
Humans
/
Factor VIII
/
Restriction Mapping
/
Genetic Testing
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Hemophilia A
Type of study:
Prognostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1996
Type:
Article
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