Demonstration of species-specific antigens of Gnathostoma spinigerum, a preliminary attempt.
Southeast Asian J Trop Med Public Health
;
1989 Jun; 20(2): 305-12
Article
in English
| IMSEAR
| ID: sea-34159
ABSTRACT
An attempt was made to demonstrate the presence of species-specific antigens for Gnathostoma spinigerum advanced third-stage larvae (GsAL3) in a rabbit receiving weekly immunization with GsAL3 for seven weeks. The homologous and heterologous antibodies against GsAL3 and G. doloresi adult worm (Gd) antigens were initially detected by immunoelectrophoresis (IEP) and ELISA after the second immunization, and their levels were gradually increased with the number of immunizations. Though cross-reactivity between GsAL3 and Gd were shown with both tests, species-specific antibodies for the homologous antigens were demonstrated. After cross-absorption of rabbit hyperimmune serum was collected after the seventh immunization, seven 'putative' species-specific precipitin bands of GsAL3 were identified. The ELISA values of the rabbit hyperimmune serum showed 50% inhibition after absorption with 0.7 micrograms/ml of homologous GsAL3 antigens as opposed to 1.0 micrograms/ml of the heterologous Gd antigens.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rabbits
/
Species Specificity
/
Thelazioidea
/
Enzyme-Linked Immunosorbent Assay
/
Immunoelectrophoresis
/
Antibodies, Helminth
/
Gnathostoma
/
Larva
/
Animals
/
Antigens, Helminth
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1989
Type:
Article
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