Serodiagnosis of human paragonimiasis caused by Paragonimus heterotremus.

Pariyanonda, S; Maleewong, W; Pipitgool, V; Wongkham, C; Morakote, N; Intapan, P; Thirakharn, B; Boonphadung, N

Pariyanonda, S; Maleewong, W; Pipitgool, V; Wongkham, C; Morakote, N; Intapan, P; Thirakharn, B; Boonphadung, N.

Southeast Asian J Trop Med Public Health ; 1990 Mar; 21(1): 103-7

Article in English | IMSEAR | ID: sea-34259

ABSTRACT

ABSTRACT

Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination tests (IHA) were evaluated for serodiagnosis of human paragonimiasis caused by Paragonimus heterotremus using homologous adult worm extract as antigen. IgG-ELISA was the most sensitive, being positive in all paragonimiasis sera tested while IHA and IgA-ELISA gave 88% and 59% positive rates respectively. Cross reactivity in IgG-ELISA was detected with fascioliasis sera, producing overall assay specificity of 97%. It is suggested that IgG-ELISA is a reliable serodiagnostic test for human paragonimiasis caused by Paragonimus heterotremus.

Subject(s)

Animals; Antibodies, Helminth/analysis; Cats; Enzyme-Linked Immunosorbent Assay; Hemagglutination Tests; Humans; Immunoglobulin A/analysis; Immunoglobulin G/analysis; Paragonimiasis/diagnosis; Paragonimus/isolation & purification

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Paragonimiasis / Paragonimus / Humans / Immunoglobulin A / Immunoglobulin G / Enzyme-Linked Immunosorbent Assay / Hemagglutination Tests / Antibodies, Helminth / Cats / Animals Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1990 Type: Article

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