Validation of ELISA test kits for detection of beta-agonist residues in meat.
Southeast Asian J Trop Med Public Health
;
2004 Jun; 35(2): 481-7
Article
in English
| IMSEAR
| ID: sea-34308
ABSTRACT
Three ELISA test kits, the Randox ELISA beta-agonist test kit, Euro-Diagnostica test kit, and Ridascreen beta-agonist test kit, were evaluated for screening of meat and liver for beta-agonist residues in fortified and field-incurred samples. It was found that the Randox beta-agonist test kit was more suitable as a screening tool due to its accuracy, ease of use, and lower cost. The tests were able to detect beta-agonist residues at the minimum level of detection, as claimed by the suppliers. The performance of the method as assessed through recovery rates of beta-agonists in fortified samples was satisfactory with a low coefficient of variation (1-3%). Repeatability, as measured through the coefficient of correlation was also satisfactory. For field-incurred positive samples, the test kit showed a sensitivity of 100% and a low rate of false positives for goat and cow tissues. However, a high rate of apparent false positives was obtained for tissues of swine.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Swine
/
Cattle
/
Enzyme-Linked Immunosorbent Assay
/
Goats
/
Drug Residues
/
Food Contamination
/
Adrenergic beta-Agonists
/
Veterinary Drugs
/
Liver
/
Animals
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2004
Type:
Article
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