Identification of clinical isolates of Leptospira spp by pulsed field gel-electrophoresis and microscopic agglutination test.

Naigowit, Pimjai; Charoenchai, Sathapana; Biaklang, Mayurachat; Seena, Umaporn; Wangroongsarb, Piyada; Sawanpanyalert, Pathom; Warachit, Paijit

Naigowit, Pimjai; Charoenchai, Sathapana; Biaklang, Mayurachat; Seena, Umaporn; Wangroongsarb, Piyada; Sawanpanyalert, Pathom; Warachit, Paijit.

Southeast Asian J Trop Med Public Health ; 2007 Jan; 38(1): 97-103

Article in English | IMSEAR | ID: sea-34410

ABSTRACT

ABSTRACT

Twenty-five clinical isolates of Leptospira spp were characterized by microscopic agglutination test (MAT) and pulsed field gel-electrophoresis (PFGE) in comparison with 23 reference Leptospira serovars and with the saprophytic L. biflexa serovar Patoc. PFGE DNA profiling was more specific and reliable than MAT.

Subject(s)

Agglutination Tests; Bacterial Typing Techniques; DNA, Bacterial/genetics; Electrophoresis, Gel, Pulsed-Field; Humans; Leptospira/classification; Leptospira interrogans/genetics; Leptospirosis/diagnosis; Reproducibility of Results; Sensitivity and Specificity; Serotyping; Thailand

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Thailand / Humans / DNA, Bacterial / Agglutination Tests / Serotyping / Reproducibility of Results / Bacterial Typing Techniques / Sensitivity and Specificity / Electrophoresis, Gel, Pulsed-Field / Leptospira Type of study: Diagnostic study / Prognostic study Country/Region as subject: Asia Language: English Journal: Southeast Asian J Trop Med Public Health Year: 2007 Type: Article

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