Development of the rapid and simple elisa (whole blood-ELISA) using coconut water-tween as a wash solution for whole blood sample from Schistosoma japonicum-infected rabbit and human.
Southeast Asian J Trop Med Public Health
;
2005 Nov; 36(6): 1383-7
Article
in English
| IMSEAR
| ID: sea-34525
ABSTRACT
PBS-Tween as a wash solution, prepared with distilled water, is used in ELISA. In areas where schistosomiasis is endemic, however, distilled water is hard to come by. We have modified a WHOLE BLOOD-ELISA test to use coconut water-Tween as a wash solution, because coconut water is easy to come by and cheap in the tropics. We applied the test to whole blood samples from rabbits and humans infected with Schistosoma japonicum. This modified WHOLE BLOOD-ELISA was confirmed to be a rapid, simple, and cost-effective method.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rabbits
/
Schistosoma japonicum
/
Schistosomiasis
/
Solutions
/
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Serologic Tests
/
Mass Screening
/
Cocos
/
Animals
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2005
Type:
Article
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