Pulsed-field gel electrophoresis as an epidemiologic tool in the investigation of laboratory acquired Salmonella typhi infection.
Southeast Asian J Trop Med Public Health
;
1997 Mar; 28(1): 82-4
Article
in English
| IMSEAR
| ID: sea-34688
ABSTRACT
Strains of Salmonella typhi implicated in two separate cases of laboratory acquired infection from patients and the medical laboratory technologists who processed the patients' samples were analysed by pulsed-field gel electrophoresis. Although all four isolates were of bacteriophage type E1, PFGE was able to demonstrate that the strains responsible for the two laboratory acquired cases were not genetically related. The PFGE patterns of the isolates from the MLTs were found to be identical to those of the corresponding patients after digestion with restriction enzyme AvrII. This provided genetic as well as epidemiological evidence for the source of the laboratory acquired infections.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Salmonella typhi
/
Typhoid Fever
/
Humans
/
Bacteriophage Typing
/
DNA Fingerprinting
/
Electrophoresis, Gel, Pulsed-Field
/
Endemic Diseases
/
Laboratory Infection
/
Malaysia
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1997
Type:
Article
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