Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.
Southeast Asian J Trop Med Public Health
;
2003 Sep; 34(3): 469-72
Article
in English
| IMSEAR
| ID: sea-34710
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Schistosoma japonicum soluble egg antigens (SEA) in un-concentrated urine was developed. The urine ELISA was applied to samples collected in a schistosomiasis-endemic village in China. The levels of anti-SEA antibodies detected in urine correlated well with those obtained with paired serum samples (r = 0.694, p<0.0001). Among 129 serum ELISA positives, 112 (86.8%) were positive by urine ELISA, while all 40 serum ELISA negatives from a non-endemic area were negative. The levels of anti-SEA in urine samples were stable up to 8 weeks of storage at 37 degrees C, with sodium azide as a preservative. Therefore, ELISA with urine samples can be used for the surveillance of schistosomiasis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Specimen Handling
/
Schistosomiasis japonica
/
Humans
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Population Surveillance
/
Sensitivity and Specificity
Type of study:
Diagnostic study
/
Evaluation studies
/
Screening study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2003
Type:
Article
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