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An experimental study on application of PCR in detection of kala-azar.
Southeast Asian J Trop Med Public Health ; 1997 Mar; 28(1): 169-72
Article in English | IMSEAR | ID: sea-34739
ABSTRACT
A polymerase chain reaction (PCR) technique was developed to detect Kala-azar. Out of 25 patients, 24 were detected, a sensitivity of 96%. The specificity rate, false positive rate, false negative rate, coincidence rate and Youdent's index were 100% (60/60), 0, 4%, 99% and 0.96 respectively. It was shown that the PCR technique can directly detect Kala-azar in samples (1 microliter) from peripheral blood.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Leishmania donovani / Humans / China / Polymerase Chain Reaction / DNA, Protozoan / Sensitivity and Specificity / Leishmaniasis, Visceral / Animals Type of study: Diagnostic study Country/Region as subject: Asia Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1997 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Leishmania donovani / Humans / China / Polymerase Chain Reaction / DNA, Protozoan / Sensitivity and Specificity / Leishmaniasis, Visceral / Animals Type of study: Diagnostic study Country/Region as subject: Asia Language: English Journal: Southeast Asian J Trop Med Public Health Year: 1997 Type: Article