NS2a-NS2b sequence analysis of DEN-4 virus strains.
Southeast Asian J Trop Med Public Health
;
2003 Sep; 34(3): 554-8
Article
in English
| IMSEAR
| ID: sea-35075
ABSTRACT
To study the genetic variability of DEN-4 Chinese isolates, and to trace the origin of DV4 Chinese isolates, we cloned and sequenced the NS2a-NS2b junction of 5 isolates and prototype DV4 (H-241). Our results show that isolates from the 1990 Guangdong epidemic, which were isolated in the early, middle, and late periods of the epidemic, share the same sequence in the NS2a-NS2b junction. The sequence similarity between isolates from the Guangdong epidemic in 1990 and DV4 H-241 is 96%; these isolates can be grouped into genotype I. The sequence similarity between the isolate from the Guangdong epidemic in 1987 and Dominica strain 814669 is 96%; this isolate can be grouped into genotype II. For the first time, our results show that there are also 2 DV4 genotypes in the Guangdong area of southern China, and these isolates perhaps were introduced from other epidemic areas outside of China.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Genetic Variation
/
Humans
/
Molecular Sequence Data
/
Base Sequence
/
Cell Line
/
China
/
Disease Outbreaks
/
Viral Nonstructural Proteins
/
DNA, Complementary
/
Dengue
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2003
Type:
Article
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