A recombinant immunodiagnostic antigen for bovine cysticercosis.
Southeast Asian J Trop Med Public Health
;
1991 Dec; 22 Suppl(): 268-70
Article
in English
| IMSEAR
| ID: sea-35407
ABSTRACT
The 70% ammonium sulfate-soluble fraction of the cyst fluid of Taenia hydatigena (designated ThFAS) was previously shown to have potential as an immunodiagnostic reagent for bovine cysticercosis. Western blot analysis indicated that the specific reactivity with antibodies in sera of T. saginata-infected cattle was associated with a 10 kDa component. Rabbit antiserum to ThFAS identified a homologous antigenic protein from the cestode Taenia crassiceps. Consequently, a cDNA expression library was constructed in lambda gt11 using poly A mRNA purified from T. crassiceps metacestodes and screened with rabbit antiserum to ThFAS. One strongly reactive clone (designated lambda TCA-2) produced a 123 kDa beta-galactosidase fusion protein which reacted in Western blot with sera from calves experimentally-infected with T. saginata and did not react with sera from uninfected calves or from cattle infected with Fasciola hepatica or with common gastrointestinal cattle parasites.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Taenia
/
Recombinant Proteins
/
DNA
/
Cattle
/
Cysticercosis
/
Antibodies, Helminth
/
Cattle Diseases
/
Gene Library
/
Blotting, Western
/
Animals
Type of study:
Prognostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1991
Type:
Article
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