Development and assessment of ELISA for serodiagnosis of HIV-infection.
Southeast Asian J Trop Med Public Health
;
1998 Dec; 29(4): 763-6
Article
in English
| IMSEAR
| ID: sea-35511
ABSTRACT
A home-made ELISA for serodiagnosis of HIV-infection was developed. It made use of the HIV viral lysate to coat on ELISA microplates. The purpose was to establish an ELISA for serodiagnosis of HIV-infection. The newly-developed ELISA, "H-ELISA", was applied to test 792 samples of HIV-positive serum as confirmed by Western blot. All 792 samples were positive by H-ELISA. It was also applied to test 540 samples of normal sera obtained from different laboratories in Srinagarind Hospital. A number 530 normal sera was negative, 8 samples were positive and confirmed by Western blot and 2 samples were false positive. It was concluded that the H-ELISA possessed 100% sensitivity with a false positive rate of 2/532(0.38%). The H-ELISA, the cost/test was less than 5 bahts, appears to be promising for substitution of imported commercial kits.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Agglutination Tests
/
Enzyme-Linked Immunosorbent Assay
/
AIDS Serodiagnosis
/
Blotting, Western
/
Sensitivity and Specificity
/
Cost Control
Type of study:
Controlled clinical trial
/
Diagnostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1998
Type:
Article
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