Molecular cloning of Plasmodium falciparum blood stage antigens and application of the recombinant proteins in serodiagnosis.
Southeast Asian J Trop Med Public Health
;
1992 Sep; 23(3): 402-5
Article
in English
| IMSEAR
| ID: sea-35567
ABSTRACT
A Plasmodium falciparum genomic DNA library was established in the expression vector lambda gt11, cloned in Escherichia coli. The library was screened with human hyperimmune sera by in situ hybridization. Twenty clones expressing P. falciparum sequences as polypeptides fused to beta-galactosidase were identified. One, CD3A/9025/60, reacted with all immune sera and expressed polypeptides that were larger than beta-galactosidase as well as reacting with antibodies to beta-galactosidase and to P. falciparum. When the fusion proteins were used as target antigens to diagnose malaria antibodies, a result was obtained which correlated well with indirect fluorescence assay.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmodium falciparum
/
Recombinant Fusion Proteins
/
Humans
/
DNA, Recombinant
/
Serologic Tests
/
Genomic Library
/
Malaria, Falciparum
/
Cloning, Molecular
/
Genes, Protozoan
/
Evaluation Studies as Topic
Type of study:
Diagnostic study
/
Evaluation studies
/
Prognostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1992
Type:
Article
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