The validation of an automated liquid chromatography system for the diagnosis of thalassemias and hemoglobinopathies.
Southeast Asian J Trop Med Public Health
;
2002 Dec; 33(4): 862-8
Article
in English
| IMSEAR
| ID: sea-35653
ABSTRACT
Accurate and precise hemoglobin separation and the quantitation of Hb A2 and Hb F are essential for the diagnosis of the thalassemias and hemoglobinopathies. Presented in this study is the validation of the the Hb A2 assay of the HbGold analyzer, a fully automated liquid chromatography system for hemoglobin separation and quantitation. Variability of Hb A2 quantitation was quite low; the CV's of within-run, between-run and interlaboratory studies were 1.8-3.1%, 3.4-6.0% and 6.8-8.8% respectively. The results of the %Hb A2 quantitated by HbGold analyzer correlated well with those given by the Bio-Rad Variant Hb testing system (r=0.98). The application of the HbGold analyzer for the diagnosis of the thalassemia phenotypes frequently observed in Thailand is considered. In conclusion, the Hb A2 assay of the HbGold analyzer could be used for the quantitation of Hb A2 and Hb F and the presumptive identification of abnormal hemoglobins.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phenotype
/
Automation
/
Thailand
/
Thalassemia
/
Humans
/
Fetal Hemoglobin
/
Hemoglobin A2
/
Hemoglobin E
/
Immunoblotting
/
Bias
Type of study:
Diagnostic study
/
Observational study
/
Risk factors
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2002
Type:
Article
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