Detection and molecular characterization of the zot gene in Vibrio cholerae and V. alginolyticus isolates.
Southeast Asian J Trop Med Public Health
;
2001 Mar; 32(1): 100-4
Article
in English
| IMSEAR
| ID: sea-35683
ABSTRACT
A total of 11 Vibrio cholerae isolates from 1996-1998 outbreaks in Malaysia and 4 V. alginolyticus were analyzed. Isolates were characterized by polymerase chain reaction (PCR) and Southern hybridization for the presence of the gene encoding zonula occludens toxin (zot). Screening of zot gene by PCR revealed the presence of this gene in V. cholerae and V. alginolyticus. The zot gene from one V. cholerae Ogawa isolate that was cloned in a pCR 2.1 TOPO vector was sequenced. The sequences obtained were 99% homologous to the zot gene sequence from the Gene Bank.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Species Specificity
/
Vibrio
/
Vibrio cholerae
/
Humans
/
DNA, Bacterial
/
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
Cholera Toxin
/
Blotting, Southern
Type of study:
Diagnostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2001
Type:
Article
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