Role of interleukin-3 and signaling pathways on beta-thalassemia/HbE erythroid progenitor cell in culture.
Southeast Asian J Trop Med Public Health
;
2007 Sep; 38(5): 897-903
Article
in English
| IMSEAR
| ID: sea-35789
ABSTRACT
In order to study the role of the cytokine interleukin-3 (IL-3) and its signaling pathways in erythropoiesis of beta-thalassemia/HbE erythroid progenitor cells, CD34 positive cells were isolated from peripheral blood of patients and healthy subjects. After culturing the cells in the presence or absence of IL-3, cell viability was measured by trypan blue staining and apoptotic cells were analyzed by flow cytometry. After 7 days of culture the highest percent erythroid progenitor cell viability was obtained with cells from healthy subjects, while the lowest percentage was found in those from splenectomized beta-thalassemia/HbE. Viability of beta-thalassemia/HbE erythroid progenitor cells in the presence of IL-3 was higher than that of nonsupplemented cells. In addition, specific inhibitors of protein kinase C (Ro-318220), phospholipase C (U-73122) and Janus kinase 2 (AG-490) were used to investigate the involvement of signaling pathways in erythropoiesis. Percent apoptosis of erythroid progenitor cells from splenectomized beta-thalassemia/HbE subjects treated with RO-318220 was higher than those of nonsplenectomized beta-thalassemia/HbE and healthy subjects. Treatment with U-73122 resulted in enhanced percent apoptotic cells from normal and beta-thalassemia/HbE subjects. All these effects were independent of IL-3 treatment.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pyrrolidinones
/
Spleen
/
Splenectomy
/
Protein Kinase C
/
Female
/
Humans
/
Male
/
Hemoglobin E
/
Signal Transduction
/
Erythroid Precursor Cells
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2007
Type:
Article
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