Effects of sonicated Prevotella intermedia, Fusobacterium nucleatum and Lactobacillus casei extracts on interleukin-8 production by human dental pulp cells.
Southeast Asian J Trop Med Public Health
;
2006 May; 37(3): 523-7
Article
in English
| IMSEAR
| ID: sea-35804
ABSTRACT
The objective of this study was to determine the effects of Prevotella intermedia, Fusobacterium nucleatum and Lactobacillus casei on the production of IL-8 by human dental pulp cells. Human dental pulp cells from teeth of young patients (aged 18-25 years) were cultured and tested with sonicated P. intermedia ATCC 25611, F. nucleatum ATCC 25586 and L. casei ATCC 4646 extracts. IL-8 secreted into the culture supernatants were measured at 6, 12 and 24 hours using a quantitative sandwich enzyme immunoassay technique. Cell viability was evaluated using trypan blue exclusion technique. IL-8 production by human dental pulp cells increased significantly at 12 and 24 hours after exposure to P. intermedia and F. nucleatum, whereas L. casei extract exhibited low IL-8 production. The sonicated bacterial extracts did not significantly affect viability or total number of dental pulp cells.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacterial Infections
/
Humans
/
Cell Survival
/
Cells, Cultured
/
Interleukin-8
/
Adolescent
/
Prevotella intermedia
/
Adult
/
Fusobacteria
/
Dental Pulp
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2006
Type:
Article
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